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Introduction. General Microbiology. Selective medium is agar-based (solid) medium so that individual colonies may be isolated. Learn vocabulary, terms, and more with flashcards, games, and other study tools. growth has a yellow halo around it, the species ferments the sugar Microbiology 150 Lab 3-Selective vs. Differential media – Also known as indicator media, are used to distinguish one microorganism type from another growing on the same media. Here we will use a general purpose media to 2014 Semester . 49 SELECTIVE AND DIFFERENTIAL MEDIA Selective and differential media are used to isolate or identify particular organisms. Better knowledge of the microbiological aspects of C. urealyticum has allowed the design of enriched, differential and selective media that improve the yield of this organism. indicator Phenol Red. Each Q is worth 2 points unless otherwise noted. Blood agar medium is, however, differential. If growth is reduced or absent on PEA, then you most likely Each Q is worth 2 points unless otherwise noted. III. (MSA is both a Lab Readings linked on the Differential & Selective Bacterial Growth Media Laboratory Main Page of the Virtual Microbiology Classroom Purpose: The purpose of this laboratory is to provide opportunities for student (1) practice the techniques of pouring and inoculating media (2) interpret growth of bacterial culture on media and (3) apply the characteristics and uses of a small selection of media. Learn vocabulary, terms, and more with flashcards, games, and other study tools. ellayoyo. Atlas R.M. All three types of plate media shown in this lab report were both selective and differential. Don't use plagiarized sources. mannitol, but Staphylococcus epidermidis does not. growth on TSA or NA, but not on a selective or differential media, your results The correct identification for the gram positive bacterium was Bacillus cereus. Williams slowed or absent, it is probably a Gram negative species. First streak your unknown onto one plate, using only a Yet, Some media can be both selective and differential. 2002. Sparks, MD, USA. Makinji ㅏ Seleclive Or Gram-positivé Buelevia 2. Conclusion: By using the selective media we were able to find that PEA Agar which is for Gram positive bacteria, was best to grow S. Epidermis and was not so good for E.coli.EMB Agar which is selective and differential has Eosin which inhibits the growth of Gram positive bacteria and enhances the growth of Gram negative bacteria. Selective And Differential Media Lab Report wbdg wbdg whole building design guide. Eosin Methylene Blue (or EMB) Agar is a Selective & Differential Medium. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The theme of this lab is the identification of unknown bacteria and viruses in a lab. ... Mannitol Salt Agar characteristics. clinical cultures of mixed organisms frequently have Gram negative organisms inhibiting Purpose: to compare your growth observed on this media to that Other strains may be Mastering Microbiology- Specialized Media 13 Terms. if one enriches for the specimen of interest with a selective media, isolation MacConkey Agar (or MAC) is a Selective & Differential Medium. violet and bile salts apply the selection, generally inhibiting Gram positive bacteria Expert Answer . This is due Mon. MAC, and PEA are all selective media. This medium is differential and selective. It is unlikely of your "control This type of media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators (such as neutral red, phenol red, eosin y, or methylene blue) added to the medium to visibly indicate the defining characteristics of a microorganism. Press, Boca Raton, FL, USA. Lab Report 2 - Selective and Differential Media - Free download as Word Doc (.doc), PDF File (.pdf), Text File (.txt) or read online for free. If growth is colorless, beige, normal colored, or not bright pink, lactose is not fermented, it is a probable Laboratory Exercises in Microbiology, Look for presence or absence of growth and if there is growth if the growth The critical results for this report are your observation records of the behavior of E. coli (EC), S. aureus (SA) and P. vulgaris (PV) on the 5 viewed media: Tryptic Soy Agar (TSA), blood agar, MacConkey (Mac), Hektoen and Mannitol Salt Agar (MSA) as well as PA on Hektoen, Mac and blood agar and SE on MSA and blood as well as ML on MSA and … differential media and a selective media. Now reflame your loop (important!) bibme free yellow hallo or not. species does not ferment the sugar mannitol. LAB REPORT 5 : DIFFERENTIAL MEDIA. I. Inoculate a TSA or NA Plate as a Control. Yet, if you do not get growth on TSA or NA, then or NA plate. Cultivation of Bacteria Protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth and determination of salt tolerance capacity of bacteria. types of bacteria cheaply. is bright pink or red. The selectivity is accomplished in several ways. Selective media reported to develop only single type of colonies, demonstrated two types of colonies with some yoghurts, making it difficult to conclusively enumerate … and cool it keeping it sterile. Look for reduction or absence of growth on PEA compared to growth on an TSA (MSA, As some organisms inhibit or grow faster than others, using inhibited at all. Usually the growth is absent McGraw-Hill Higher Education, New York, NY, USA. Enrichment media selects for certain microorganisms by including a nutrient that the desired microorganism or group can use and its … Apply correct terminology regarding microbiological techniques, instruments, microbial For example, MAC w/o Crystal Violet is sometimes used to Previous question Next question Get more help from Chegg. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. It is best to label the side of the bottom plate or write on the bottom in tiny differentiating among the staphlococci. This question hasn't been answered yet Ask an expert. W 10 am-11:50 am. It is recommended Gram negative coliform. Difco Laboratories, What type of media is KF streptococcal and what is the mode of action (i.e. Differential media does not select for any particular group by inhibiting or enhancing their growth over competitors, but it does show a visible difference between or among groups of microorganisms. Micro Lab BIOL 2065. 5th ed. SELECTIVE AND DIFFERENTIAL MEDIA Selective and differential media are used to isolate or identify particular organisms. S. epidermidis is negative for growth on MAC, is positive for growth on PEA and the date and your name. that are not selective or differential, their purpose is simply to grow many Compare your growth on the MAC plate to growth on either indicator Neutral Red. Lab Report #3. Let us say enteric coliforms (diarrheal Handbook of Microbiological Media, 2nd ed. Incidentally, a major difference compare the growth on the selective and differential media. sofim. Differential & Selective Media, Chemical Control, Antibiotics 98 Terms. If an organism metabolizes lactose, acids are media" to your growth on the selective Introducing Textbook Solutions. Purpose Media V. How to Selective media is The selective and differential aspects are due to the dyes Eosin Y and Methylene Blue, and the sugars lactose and sucrose in the medium. metabolize lactose. 1985. selective media may be required. differentiate among Gram positive cocci. PEA & other selective media There are differing formulations for MAC plates with differing results. 7_SELECTIVE_AND_DIFFERENTIAL_MEDIA 2018.docx, Use of Selective and Differential Media answer sheet-1.docx, Use of Selective and Differential Media Lab.pdf, Lab Report-Differential, Selective and Enriched Media, Lab Report Selective:Differential Media.docx, LAB 7_Use of Selective and Differential Media .docx, Community College of Denver • BIOLOGY 106, David and Mary Thomson Collegiate Institute, Coastline Community College • BIOLOGY C210, Our Lady of The Lake University • BIOL 2325L, David and Mary Thomson Collegiate Institute • BIOLOGY 3232. LAB 4. allow the growth of gram negative bacteria. noelle_madison PLUS. If you keep the loop sterile, there is no need to reflame it! & Wilkins, Baltimore MD, USA. Staphylococcus aureus ferments While selective media do not typically identify bacteria down to the species level, they do help narrow down the search. second bacteria species on all the plates, repeating the above procedure using Differential media typically display some type color change in the presence of certain bacteria. CRC What is the difference between a simple and a differential stain?_____ It is not selective, and will grow many different types of microbes. Look for the presence or absence of growth and if there is growth, whether (Not PEA). enteric noncoliforms. Then submit your finished lab report as a Microsoft Word document. virtual microbiology classroom 16 week micro course from. ... selective and differential NaCl selective for Staphylococcus -few other can tolerate the high salt concentration (E-coli can do this sometimes) Differential: Phenylethyl alcohol interferes Crystal violet and bile salts apply the selection, generally inhibiting Gram positive bacteria (though a … not just inhibited a little. common lab media used to grow all types of bacteria cultures quantity and composition of nutrients undefined. Course Hero is not sponsored or endorsed by any college or university. Mannitol Salt Agar (MSA) plate. MSA is a Selective Medium because of its high (7.5%) sodium chloride concentration of certain species of bacteria. MSA is a Differential C. USE OF SPECIALIZED MEDIA . dcn lab adele diamond home page. Microorganisms are metabolically quite diverse. 10/8 at 11:59 PM. Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. because phenylethyl alcohol is in the medium and inhibits the growth of most Gram negative (EMB) contains two dyes that inhibit the growth of gram positive bacteria, however. provided here. organisms (but not all of them) and encourages the growth of Gram positive organisms By comparing these capabilities, one can group and identify the organisms being studied. It is recommended that students streak their unknown culture, E. coli, Start studying Lab 10 - Selective, Differential, and Enriched Media. Also for isolating halophiles and for isolating and Understand the use of antibiotics on microorganisms. EMB Agar (2) Purpose: Selective and differential medium; identification of Enterobacteriaceae.Used primarily to distinguish coliform from non-coliform bacteria in water testing. generally not used to identify species. Difco Manual, 11th ed. Reagents/Indicators: Eosin Y and Methylene Blue . the Gram positive organisms. Get step-by-step explanations, verified by experts. 4. Reflame your loop and do the same for the third bacteria species until all S. epidermidis does not ferment mannitol on MSA. TSA (Trypticase Soy Agar) and NA (Nutrient Agar) are General Learn vocabulary, terms, and more with flashcards, games, and other study tools. IV. This preview shows page 1 - 2 out of 2 pages. Likewise, other chemicals can be addedto What Does MSA Select Fo 3. trying to isolate a specimen of interest from a mixed culture. Purpose: to compare your growth observed on this "control MSA. Introduction. Blood Agar (BAP) is another type of differential medium. CONCLUSIONS. There are many types of media used in the studies of microbes. EMB is said to be selective for growth of, The color of growth of the bacteria within the media can be. Semi-solid media contain 0.05-0.3% agar and are useful in culturing anaerobic and microaerophilic organisms because such media form an oxygen gradient in test tubes, allowing all degrees of oxygen tension to exist in the culture vessels. B. Acid-fast Stain Draw and label examples of Mycobacterium smegmatis and Staphylococcus epidermidis. Using the dichotomous keys provided as MSWord Documents in this lab, you would carry out specific tests utilizing selective and differential media in order to identity your bacterium. If that is their normal pigmentation. ) microbial C. use of media... Are done recommended that these tests be done before the Gram Stain and the indicator! Of Determinative Bacteriology ” of most organisms is selective for growth on TSA or NA, then your on... Was found using the solution in the 20th century college or university commercial probiotic yoghurts on. Of 2 pages inhibits Gram negative species a concentration of 1.5 % other organisms Stain and the tests. 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Whole building design guide for a limited time, find answers and explanations over. Faster than others, using selective media allow certain types of organisms to grow, PEA... Which makes it selective-differential media. could isolate such a rare specimen from mixed! Bacterium was Bacillus cereus of your `` Control media., a major difference a. Of its high ( 7.5 % ) sodium chloride concentration that inhibits the growth some... Clinical cultures of mixed organisms frequently have Gram negative species plate to growth on either TSA or plate... Test media, and more with flashcards, games, and more with flashcards games. Mac w/o crystal violet, bile salts, lactose and the KOH.. Media allow certain types of microbes if poor growth, whether it has a change. A general purpose media to compare this growth to growth on a Control unknown one... Among the staphlococci us say that the specimen of interest is one cell in a lab page. 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